House Primers

Listed Below are the primers that Arizona Genetics Core will supply free of charge. Please check the sequence against that of your vector to verify the sequence.

NOTE: Please type these primer names in uppercase when submitting your samples online for sequencing

  • KS: CCT-CgA-ggT-CgA-Cgg-TAT-Cg
  • M13F: TgT-AAA-ACg-ACG-gCC-AgT
  • M13R: Agg-AAA-CAg-CTA-TgA-CCA-Tg
  • PCM13F*: CgC-CAg-ggT-TTT-CCC-AgT-CAC-gAC
  • PCM13R*: TCA-CAC-Agg-AAA-CAg-CTA-TgA-C
  • SK: CCg-CTC-TAg-AAC-TAg-Tgg-ATC
  • SP6: TTT-Agg-TgA-CAC-TAT-AgA-ATA
  • T3: AAT-TAA-CCC-TCA-CTA-AAg-gg
  • T7: TAA-TAC-gAC-TCA-CTA-TAg-gg

*PCM13F and PCM13R are the same as pUCM13F and pUCM13R